Molecular Cloning and Expression of Cucumisin (Cuc m 1), a Subtilisin-like Protease of Cucumis melo in Escherichia coli
نویسندگان
چکیده
منابع مشابه
Molecular Cloning, Characterization, and Expression of Cuc m 2, a Major Allergen in Cucumis melo
Background: Several studies reported the clinical features of IgE-mediated hypersensitivity after ingestion of melon. Melon allergy is a common IgE-mediated fruit allergy in Iran. This prompted us to investigate immunochemical and molecular properties of the major allergen in melon fruit, to compare the IgE-binding capacity of the natural protein with the recombinant allergen, and to determine ...
متن کاملMolecular cloning, characterization, and expression of Cuc m 2, a major allergen in Cucumis melo.
BACKGROUND Several studies reported the clinical features of IgE-mediated hypersensitivity after ingestion of melon. Melon allergy is a common IgE-mediated fruit allergy in Iran. This prompted us to investigate immunochemical and molecular properties of the major allergen in melon fruit, to compare the IgE-binding capacity of the natural protein with the recombinant allergen, and to determine c...
متن کاملmolecular cloning, characterization, and expression of cuc m 2, a major allergen in cucumis melo
background: several studies reported the clinical features of ige-mediated hypersensitivity after ingestion of melon. melon allergy is a common ige-mediated fruit allergy in iran. this prompted us to investigate immunochemical and molecular properties of the major allergen in melon fruit, to compare the ige-binding capacity of the natural protein with the recombinant allergen, and to determine ...
متن کاملIdentification and Molecular Characterization of the cDNA Encoding Cucumis melo Allergen, Cuc m 3, a Plant Pathogenesis-Related Protein
Background: Melon (Cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. To date, two allergen molecules, Cuc m 1 and Cuc m 2, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of Cuc m 3. Methods:The Cuc m 3 cDNA has been characterized by rapid amplification of cDNA ends (RACE), which ...
متن کاملIdentification and molecular characterization of the cDNA encoding Cucumis melo allergen, Cuc m 3, a plant pathogenesis-related protein.
BACKGROUND Melon (Cucumis melo) allergy is one of the most common food allergies, characterized by oral allergy syndrome. To date, two allergen molecules, Cuc m 1 and Cuc m 2, have been fully characterized in melon pulp, but there are few reports about the molecular characteristics of Cuc m 3. METHODS The Cuc m 3 cDNA has been characterized by rapid amplification of cDNA ends (RACE), which re...
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ژورنال
عنوان ژورنال: Allergology International
سال: 2011
ISSN: 1323-8930
DOI: 10.2332/allergolint.10-oa-0195